MRI Relaxometry for Quantitative Analysis of USPIO Uptake in Cerebral Small Vessel Disease.

ABSTRACT: A protocol for evaluating ultrasmall superparamagnetic particles of iron oxide (USPIO) uptake and elimination in cerebral small vessel disease patients was developed and piloted. B?-insensitive R? measurement was evaluated in vitro. Twelve participants with history of minor stroke were scanned at 3-T MRI including structural imaging, and R? and R?* mapping. Participants were scanned (i) before and (ii) after USPIO (ferumoxytol) infusion, and again at (iii) 24?30 h and (iv) one month. Absolute and blood-normalised changes in R? and R?* were measured in white matter (WM), deep grey matter (GM), white matter hyperintensity (WMH) and stroke lesion regions. R? measurements were accurate across a wide range of values. R? (p < 0.05) and R?* (p < 0.01) mapping detected increases in relaxation rate in all tissues immediately post-USPIO and at 24?30 h. R?* returned to baseline at one month. Blood-normalised R? and R?* changes post-infusion and at 24?30 h were similar, and were greater in GM versus WM (p < 0.001). Narrower distributions were seen with R?* than for R? mapping. R? and R?* changes were correlated at 24?30 h (p < 0.01). MRI relaxometry permits quantitative evaluation of USPIO uptake; R?* appears to be more sensitive to USPIO than R?. Our data are explained by intravascular uptake alone, yielding estimates of cerebral blood volume, and did not support parenchymal uptake. Ferumoxytol appears to be eliminated at 1 month. The approach should be valuable in future studies to quantify both blood-pool USPIO and parenchymal uptake associated with inflammatory cells or blood-brain barrier leak.

SUBMITTER: Thrippleton MJ 

PROVIDER: S-EPMC6387454 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature