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Protein refractive index increment is determined by conformation as well as composition.

ABSTRACT: The refractive index gradient of the eye lens is controlled by the concentration and distribution of its component crystallin proteins, which are highly enriched in polarizable amino acids. The current understanding of the refractive index increment ([Formula: see text]) of proteins is described using an additive model wherein the refractivity and specific volume of each amino acid type contributes according to abundance in the primary sequence. Here we present experimental measurements of [Formula: see text] for crystallins from the human lens and those of aquatic animals under uniform solvent conditions. In all cases, the measured values are much higher than those predicted from primary sequence alone, suggesting that structural factors also contribute to protein refractive index.

SUBMITTER: Khago D

PROVIDER: S-EPMC6387658 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Protein refractive index increment is determined by conformation as well as composition.

Khago Domarin D Bierma Jan C JC Roskamp Kyle W KW Kozlyuk Natalia N Martin Rachel W RW

Journal of physics. Condensed matter : an Institute of Physics journal 20181003 43

The refractive index gradient of the eye lens is controlled by the concentration and distribution of its component crystallin proteins, which are highly enriched in polarizable amino acids. The current understanding of the refractive index increment ([Formula: see text]) of proteins is described using an additive model wherein the refractivity and specific volume of each amino acid type contributes according to abundance in the primary sequence. Here we present experimental measurements of [Form ...[more]

PMID: 30280702

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