Dynamic Culture Substrates That Mimic the Topography of the Epidermal-Dermal Junction.
Ontology highlight
ABSTRACT: Impact statement
In human skin the junction between the epidermis and dermis undulates. Epidermal stem cells pattern according to their position relative to those undulations. Here we describe a rig in which epidermal cells are cultured on a collagen-coated poly(d,l-lactide-co-glycolide) (PLGA) membrane. When a vacuum is applied the membrane is induced to undulate. Stem cells cluster in response to the vacuum, whereas differentiating cells do not. Rho kinase inhibition results in loss of clustering, suggesting a role for Rho family members in the process. This dynamic platform is a new tool for investigating changes in the skin with age and disease.
SUBMITTER: Helling AL
PROVIDER: S-EPMC6388717 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Publications
Dynamic Culture Substrates That Mimic the Topography of the Epidermal-Dermal Junction.
Helling Ayelen L AL Viswanathan Priyalakshmi P Cheliotis Katerina S KS Mobasseri Seyedeh Atefeh SA Yang Ying Y El Haj Alicia J AJ Watt Fiona M FM
Tissue engineering. Part A 20181228 3-4
<h4>Impact statement</h4>In human skin the junction between the epidermis and dermis undulates. Epidermal stem cells pattern according to their position relative to those undulations. Here we describe a rig in which epidermal cells are cultured on a collagen-coated poly(d,l-lactide-co-glycolide) (PLGA) membrane. When a vacuum is applied the membrane is induced to undulate. Stem cells cluster in response to the vacuum, whereas differentiating cells do not. Rho kinase inhibition results in loss of ...[more]
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