Arginine Side-Chain Hydrogen Exchange: Quantifying Arginine Side-Chain Interactions in Solution.

ABSTRACT: The rate with which labile backbone hydrogen atoms in proteins exchange with the solvent has long been used to probe protein interactions in aqueous solutions. Arginine, an essential amino acid found in many interaction interfaces, is capable of an impressive range of interactions via its guanidinium group. The hydrogen exchange rate of the guanidinium hydrogens therefore becomes an important measure to quantify side-chain interactions. Herein we present an NMR method to quantify the hydrogen exchange rates of arginine side-chain ¹ H^ϵ protons and thus present a method to gauge the strength of arginine side-chain interactions. The method employs ¹³ C-detection and the one-bond deuterium isotope shift observed for ¹⁵ N^ϵ to generate two e

SUBMITTER: Mackenzie HW 

PROVIDER: S-EPMC6391956 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature