Cytoprotective effects of Avenathramide C against oxidative and inflammatory stress in normal human dermal fibroblasts.
Ontology highlight
ABSTRACT: Natural polyphenols are promising anti-aging compounds not only for their antioxidant activity, but also their ability to activate specific cellular pathways mediating the aging process. Avenanthramide C (Avn C), found exclusively in oats, is a natural antioxidant associated with free radical scavenging; however, it is how this compound elicits other protective effects. We investigated the intracellular antioxidant activity of Avn C and other cytoprotective potential in normal human skin fibroblasts exposed to extracellular stress. Avn C reduced H2O2-induced oxidative stress by reducing intracellular free radical levels and antioxidant gene transcripts. Avn C also resulted in decreased levels of gene transcripts encoding pro-inflammatory cytokines in response to H2O2 or tumor necrosis factor-? (TNF-?). This reduction in cytokine gene transcription occurred concomitantly with reduced phosphorylated nuclear factor-?B (NF-?B) p65, and decreased NF-?B DNA binding. Avn C further induced heme oxygense-1 (HO-1) expression through increased Nrf2 DNA binding activity, demonstrating a second mechanism by which Avn C attenuates cellular stress. Collectively, our findings indicate that Avn C protects normal human skin fibroblasts against oxidative stress and inflammatory response through NF-?B inhibition and Nrf2/HO-1 activation.
SUBMITTER: Wang C
PROVIDER: S-EPMC6393498 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
ACCESS DATA