Dataset Information

Draft Genome Sequencing of the Pathogenic Fungus Cladosporium phlei ATCC 36193 Identifies Candidates of Novel Polyketide Synthase Genes Involved in Perylenequinone-Group Pigment Production.

ABSTRACT: Cladosporium phlei, which causes purple eyespot disease, has been focused on as a source of phleichrome from the perylenequinone group of pigments. Although this agent is important in photodynamic therapy, there are no genome sequences for the species. Here, we sequenced the genome of C. phlei and reported the draft sequence. The total length of the draft genome was approximately 31.8?Mb, and 9571 genes were predicted. Phylogenetic analysis showed that Cladosporium sphaerospermum, Rachicladosporium sp., and Rachicladosporium antarcticum were closely related, and this result corresponded to the taxonomic data. In addition to the draft genome sequence, we report four candidates of new polyketide synthase (PKS) genes, involved in the production of perylenequinone-group pigments.

SUBMITTER: Kang YN

PROVIDER: S-EPMC6393816 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Draft Genome Sequencing of the Pathogenic Fungus Cladosporium phlei ATCC 36193 Identifies Candidates of Novel Polyketide Synthase Genes Involved in Perylenequinone-Group Pigment Production.

Kang Yu-Na YN So Kum-Kang KK Kim Do-Wan DW Kim Dae-Hyuk DH Lee Tae-Ho TH

Evolutionary bioinformatics online 20190226

Cladosporium phlei, which causes purple eyespot disease, has been focused on as a source of phleichrome from the perylenequinone group of pigments. Although this agent is important in photodynamic therapy, there are no genome sequences for the species. Here, we sequenced the genome of C. phlei and reported the draft sequence. The total length of the draft genome was approximately 31.8 Mb, and 9571 genes were predicted. Phylogenetic analysis showed that Cladosporium sphaerospermu ...[more]

PMID: 30833809

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Project description:Pseudocercospora fijiensis, causal agent of black Sigatoka of banana, produces polyketide synthase (PKS) pathways shown to be important in disease development by related Dothideomycete fungi. Genome analysis of the P. fijiensis PKS8-1 gene identified it as part of a gene cluster including genes encoding two transcription factors, a regulatory protein, a glyoxylase/beta-lactamase-like protein, an MFS transporter, a cytochrome P450, two aldo/keto reductases, a dehydrogenase, and a decarboxylase. Genome analysis of the related pathogens Pseudocercospora musae, Pseudocercospora eumusae, and Pseudocercospora pini-densiflorae, identified orthologous clusters containing a nearly identical combination of genes. Phylogenetic analysis of PKS8-1 identified homology to PKS proteins in the monodictyphenone and cladofulvin pathways in Aspergillus nidulans and Cladosporium fulvum, respectively. Analysis of clustered genes showed that the PKS8-1 cluster shares genes for enzymes involved in the production of the emodin intermediate in the monodictyphenone and cladofulvin pathways, but differs in many genes, suggesting production of a different metabolic product. Time course analysis of gene expression in infected banana showed up-regulation of PKS8-1 and four of eight clustered genes as early as 2 weeks post-inoculation and remaining high through 9 weeks. Overexpression of the pathway through constitutive expression of an aflR-like transcription factor gene in the cluster resulted in increased expression in culture of PKS8-1 as well as the four clustered genes that are up-regulated in infected plants. No differences were seen in timing or severity of disease symptoms with the overexpression strains relative to controls, however gene expression analysis showed no difference in expression in planta by an overexpression strain relative to controls. Thus constitutive expression of the aflR-like gene is not sufficient to upregulate the pathway above normal expression in planta. Pathway expression during all phases of disease development and conservation of the pathway in related Pseudocercospora species support a role for this pathway in disease.

Dataset Information

Draft Genome Sequencing of the Pathogenic Fungus Cladosporium phlei ATCC 36193 Identifies Candidates of Novel Polyketide Synthase Genes Involved in Perylenequinone-Group Pigment Production.

Publications

Draft Genome Sequencing of the Pathogenic Fungus <i>Cladosporium phlei</i> ATCC 36193 Identifies Candidates of Novel Polyketide Synthase Genes Involved in Perylenequinone-Group Pigment Production.

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