A novel approach to label bone marrow-derived mesenchymal stem cells with mixed-surface PAMAM dendrimers.

ABSTRACT: BACKGROUND:Transplantation of mesenchymal stem cells has created enormous opportunities as a potential treatment for various diseases including neurodegenerative diseases. Given current techniques, such as Hoechst labeling, have safety and leakage issues, our study focused, as a proof-of-concept, on a new dendrimer-based technique for labeling these stem cells to ensure their efficacy and safety following transplantation into the brain of a healthy mice. METHODS AND RESULTS:The bone marrow-derived mesenchymal stem cells (BM-MSCs) were labeled using polyaminoamine (PAMAM) dendrimers following which their stemness based on their proliferation and differentiation ability were analyzed by gold standard methods. These labeled BM-MSCs were transplanted into the striatum of C57BL/6J mice and were

SUBMITTER: Munro N 

PROVIDER: S-EPMC6393977 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature