Comparing DNA methylation profiles in saliva and intestinal mucosa.

ABSTRACT: BACKGROUND:Altered epigenetic profiles are a feature of intestinal diseases, including ulcerative colitis and Crohn's disease. DNA methylation studies in these diseases have utilised intestinal mucosal tissue or blood which can be difficult to collect, particularly for large-scale research studies. Saliva is an attractive alternative for epigenetic studies as it is easy to collect and provides high quality methylation profiles. The aim of the study was to determine the utility of saliva as an alternative for DNA methylation studies of intestinal disorders. RESULTS:DNA methylation in saliva and intestinal mucosa samples were compared in individuals (n =?10) undergoing endoscopies using the Illumina Infinium Methylation 450?K Beadchip array. We found that DNA methylation was correlated between tissue types within an individual (Pearson correlation co-efficient r?=?0.92 to 0.95, p 

SUBMITTER: Hearn NL 

PROVIDER: S-EPMC6394071 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature