Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR.
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ABSTRACT:
SUBMITTER: Marvin JS
PROVIDER: S-EPMC6394230 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Publications
Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR.
Marvin Jonathan S JS Scholl Benjamin B Wilson Daniel E DE Podgorski Kaspar K Kazemipour Abbas A Müller Johannes Alexander JA Schoch Susanne S Quiroz Francisco José Urra FJU Rebola Nelson N Bao Huan H Little Justin P JP Tkachuk Ariana N AN Cai Edward E Hantman Adam W AW Wang Samuel S-H SS DePiero Victor J VJ Borghuis Bart G BG Chapman Edwin R ER Dietrich Dirk D DiGregorio David A DA Fitzpatrick David D Looger Loren L LL
Nature methods 20181030 11
Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spine ...[more]
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