Increases in cytosolic Ca²⁺ induce dynamin- and calcineurin-dependent internalisation of CFTR.

ABSTRACT: The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated, apical anion channel that regulates ion and fluid transport in many epithelia including the airways. We have previously shown that cigarette smoke (CS) exposure to airway epithelia causes a reduction in plasma membrane CFTR expression which correlated with a decrease in airway surface hydration. The effect of CS on CFTR was dependent on an increase in cytosolic Ca²⁺. However, the underlying mechanism for this Ca²⁺-dependent, internalisation of CFTR is unknown. To gain a better understanding of the effect of Ca²⁺ on CFTR, we performed whole cell current recordings to study the temporal effect of raising cytosolic Ca²⁺ on CFTR function. We show that an increase in c

SUBMITTER: Patel W 

PROVIDER: S-EPMC6394554 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature