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IL-1? activation in response to Staphylococcus aureus lung infection requires inflammasome-dependent and independent mechanisms.


ABSTRACT: Maintaining balanced levels of IL-1? is extremely important to avoid host tissue damage during infection. Our goal was to understand the mechanisms behind the reduced pathology and decreased bacterial burdens in Ifnlr1-/- mice during lung infection with Staphylococcus aureus. Intranasal infection of Ifnlr1-/- mice with S. aureus led to significantly improved bacterial clearance, survival and decrease of proinflammatory cytokines in the airway including IL-1?. Ifnlr1-/- mice treated with recombinant IL-1? displayed increased bacterial burdens in the airway and lung. IL-1? levels in neutrophils from Ifnlr1-/- infected mice lungs were decreased when compared to neutrophils from WT mice. Mice lacking NLRP3 and caspase-1 had reduced IL-1? levels 4 h after infection, due to reductions or absence of active caspase-1 respectively, but levels at 24 h were comparable to WT infected mice. Ifnlr1-/- infected mice had decreases in both active caspase-1 and neutrophil elastase indicating an important role for the neutrophil serine protease in IL-1? processing. By inhibiting neutrophil elastase, we were able to decrease IL-1? levels by 39% in Nlrp3-/- infected mice when compared to WT mice. These results highlight the crucial role of both proteases in IL-1? processing, via inflammasome-dependent and -independent mechanisms.

SUBMITTER: Pires S 

PROVIDER: S-EPMC6394835 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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IL-1β activation in response to Staphylococcus aureus lung infection requires inflammasome-dependent and independent mechanisms.

Pires Sílvia S   Parker Dane D  

European journal of immunology 20180819 10


Maintaining balanced levels of IL-1β is extremely important to avoid host tissue damage during infection. Our goal was to understand the mechanisms behind the reduced pathology and decreased bacterial burdens in Ifnlr1<sup>-/-</sup> mice during lung infection with Staphylococcus aureus. Intranasal infection of Ifnlr1<sup>-/-</sup> mice with S. aureus led to significantly improved bacterial clearance, survival and decrease of proinflammatory cytokines in the airway including IL-1β. Ifnlr1<sup>-/-  ...[more]

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