Ontology highlight
ABSTRACT:
SUBMITTER: Mussil B
PROVIDER: S-EPMC6395610 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Mussil Bianka B Suspène Rodolphe R Caval Vincent V Durandy Anne A Wain-Hobson Simon S Vartanian Jean-Pierre JP
Scientific reports 20190228 1
Human cells are stressed by numerous mechanisms that can lead to leakage of mitochondrial DNA (mtDNA) to the cytoplasm and ultimately apoptosis. This agonist DNA constitutes a danger to the cell and is counteracted by cytoplasmic DNases and APOBEC3 cytidine deamination of DNA. To investigate APOBEC3 editing of leaked mtDNA to the cytoplasm, we performed a PCR analysis of APOBEC3 edited cytoplasmic mtDNA (cymtDNA) at the single cell level for primary CD4<sup>+</sup> T cells and the established P2 ...[more]