Dataset Information

Injectable Slippery Lubricant-Coated Spiky Microparticles with Persistent and Exceptional Biofouling-Resistance.

ABSTRACT: Injectable micron-sized particles have historically achieved promising applications, but they continued to suffer from long-term biofouling caused by the adhesions of biomolecules, cells, and bacteria. Recently, a slippery lubricant infusion porous substrate (SLIPS) exhibited robust antiadhesiveness against many liquids; however, they were constructed using a 2D substrate, and they were not suitable for in vivo applications, such as injectable biomaterials. Inspired by SLIPS, here, we report the first case of injectable solid microparticles coated with a lubricating liquid surface to continuously resist biofouling. In our design, microparticles were attached with nanospikes and fluorinated to entrap the lubricant. The nanospikes enabled the lubricant-coated spiky microparticles (LCSMPs) to anomalously disperse in water despite the attraction between the surfaces of the microparticles. This result indicated that the LCSMPs exhibited persistent anomalous dispersity in water while maintaining a robust lubricating surface layer. LCSMPs prevented the adhesion of proteins, mammalian cells, and bacteria, including Escherichia coli and Staphylococcus aureus. LCSMPs also reduced in vivo fibrosis while conventional microparticles were heavily biofouled. This technology introduced a new class of injectable anti-biofouling microparticles with reduced risks of inflammation and infections.

SUBMITTER: Yang C

PROVIDER: S-EPMC6396194 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Injectable Slippery Lubricant-Coated Spiky Microparticles with Persistent and Exceptional Biofouling-Resistance.

Yang Chengduan C He Gen G Zhang Aihua A Wu Qianni Q Zhou Lingfei L Hang Tian T Liu Di D Xiao Shuai S Chen Hui-Jiuan HJ Liu Fanmao F Li Linxian L Wang Ji J Xie Xi X

ACS central science 20190114 2

Injectable micron-sized particles have historically achieved promising applications, but they continued to suffer from long-term biofouling caused by the adhesions of biomolecules, cells, and bacteria. Recently, a slippery lubricant infusion porous substrate (SLIPS) exhibited robust antiadhesiveness against many liquids; however, they were constructed using a 2D substrate, and they were not suitable for <i>in vivo</i> applications, such as injectable biomaterials. Inspired by SLIPS, here, we rep ...[more]

PMID: 30834313

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Project description:IntroductionReducing the protein adsorption of nanoparticles (NPs) as drug carriers to slow their rapid clearance by macrophages uptake is a critical challenge for NPs clinical translational applications. Despite extensive research efforts to inhibit cellular uptake, including covering biological agents or surface chemical coatings to impart "stealth" properties to NPs, their stability remains insufficient.ObjectivesDeveloped a novel surface modification technology based on a physical infusion engineering approach to achieve persistent inhibition of protein adhesion and cellular uptake by nanocarriers.MethodsThe nanoparticles were prepared based on conventional drug carrier mesoporous silica NPs through a two-step process. A functional nanoscale slippery surface was formed by grafting "liquid-like" brushes on the particles surface, and then a lubricant-entrenched slippery surfaces (LESS) was formed by infusing silicone oil lubricant into the entire surface. Co-incubation with macrophages (in vitro and in vivo) was used to examine the anti-uptake properties of modified NPs. The anti-adhesion properties of LESS coating surfaces to various liquids, proteins and cells were used to analyze the anti-uptake mechanism. Loaded with drugs, combined with tumor models, to evaluate the drug utilization of modified NPs.ResultsRelying on the stable and slippery LESS coating, the modified surface could prevent the adhesion of various liquids and effectively shield against the adhesion of proteins and cells, as well as remarkably reduce macrophage cellular uptake in vitro and in vivo. In addition, the LESS coating does not affect cell activity and allows NPs to be loaded with drugs, significantly improving the utilization of drugs in vitro and in vivo. This allows the NPs to reach to the target tumor site for drug delivery without active clearance by macrophages.ConclusionOur research introduces a new nanocarrier technology to improve anti-biofouling performance and stealth efficiency that will facilitate the development of nanomedicines for clinical transformation applications.

Project description:Young's equation is fundamental to the concept of the wettability of a solid surface. It defines the contact angle for a droplet on a solid surface through a local equilibrium at the three-phase contact line. Recently, the concept of a liquid Young's law contact angle has been developed to describe the wettability of slippery liquid-infused porous surfaces (SLIPS) by droplets of an immiscible liquid. In this work, we present a new method to fabricate biphilic SLIP surfaces and show how the wettability of the composite SLIPS can be exploited with a macroscopic wedge-shaped pattern of two distinct lubricant liquids. In particular, we report the development of composite liquid surfaces on silicon substrates based on lithographically patterning a Teflon AF1600 coating and a superhydrophobic coating (Glaco Mirror Coat Zero), where the latter selectively dewets from the former. This creates a patterned base surface with preferential wetting to matched liquids: the fluoropolymer PTFE with a perfluorinated oil Krytox and the hydrophobic silica-based GLACO with olive oil (or other mineral oils or silicone oil). This allows us to successively imbibe our patterned solid substrates with two distinct oils and produce a composite liquid lubricant surface with the oils segregated as thin films into separate domains defined by the patterning. We illustrate that macroscopic wedge-shaped patterned SLIP surfaces enable low-friction droplet self-propulsion. Finally, we formulate an analytical model that captures the dependence of the droplet motion as a function of the wettability of the two liquid lubricant domains and the opening angle of the wedge. This allows us to derive scaling relationships between various physical and geometrical parameters. This work introduces a new approach to creating patterned liquid lubricant surfaces, demonstrates long-distance droplet self-propulsion on such surfaces, and sheds light on the interactions between liquid droplets and liquid surfaces.

Dataset Information

Injectable Slippery Lubricant-Coated Spiky Microparticles with Persistent and Exceptional Biofouling-Resistance.

Publications

Injectable Slippery Lubricant-Coated Spiky Microparticles with Persistent and Exceptional Biofouling-Resistance.

Similar Datasets

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