Semi-automated fact-checking of nucleotide sequence reagents in biomedical research publications: The Seek & Blastn tool.

ABSTRACT: Nucleotide sequence reagents are verifiable experimental reagents in biomedical publications, because their sequence identities can be independently verified and compared with associated text descriptors. We have previously reported that incorrectly identified nucleotide sequence reagents are characteristic of highly similar human gene knockdown studies, some of which have been retracted from the literature on account of possible research fraud. Because of the throughput limitations of manual verification of nucleotide sequences, we developed a semi-automated fact checking tool, Seek & Blastn, to verify the targeting or non-targeting status of published nucleotide sequence reagents. From previously described and unknown corpora of 48 and 155 publications, respectively, Seek & Blastn correc

SUBMITTER: Labbe C 

PROVIDER: S-EPMC6396917 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature