Rapid Sequencing of Multiple RNA Viruses in Their Native Form.
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ABSTRACT:
SUBMITTER: Wongsurawat T
PROVIDER: S-EPMC6398364 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Rapid Sequencing of Multiple RNA Viruses in Their Native Form.
Wongsurawat Thidathip T Jenjaroenpun Piroon P Taylor Mariah K MK Lee Jasper J Tolardo Aline Lavado AL Parvathareddy Jyothi J Kandel Sangam S Wadley Taylor D TD Kaewnapan Bualan B Athipanyasilp Niracha N Skidmore Andrew A Chung Donghoon D Chaimayo Chutikarn C Whitt Michael M Kantakamalakul Wannee W Sutthent Ruengpung R Horthongkham Navin N Ussery David W DW Jonsson Colleen B CB Nookaew Intawat I
Frontiers in microbiology 20190225
Long-read nanopore sequencing by a MinION device offers the unique possibility to directly sequence native RNA. We combined an enzymatic poly-A tailing reaction with the native RNA sequencing to (i) sequence complex population of single-stranded (ss)RNA viruses in parallel, (ii) detect genome, subgenomic mRNA/mRNA simultaneously, (iii) detect a complex transcriptomic architecture without the need for assembly, (iv) enable real-time detection. Using this protocol, positive-ssRNA, negative-ssRNA, ...[more]
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