Ontology highlight
ABSTRACT:
SUBMITTER: Slesarev A
PROVIDER: S-EPMC6401131 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Slesarev Alexei A Viswanathan Lakshmi L Tang Yitao Y Borgschulte Trissa T Achtien Katherine K Razafsky David D Onions David D Chang Audrey A Cote Colette C
Scientific reports 20190305 1
The robust detection of structural variants in mammalian genomes remains a challenge. It is particularly difficult in the case of genetically unstable Chinese hamster ovary (CHO) cell lines with only draft genome assemblies available. We explore the potential of the CRISPR/Cas9 system for the targeted capture of genomic loci containing integrated vectors in CHO-K1-based cell lines followed by next generation sequencing (NGS), and compare it to popular target-enrichment sequencing methods and to ...[more]