A versatile image analysis platform for three-dimensional nuclear reconstruction.
Ontology highlight
ABSTRACT:
SUBMITTER: Williams JF
PROVIDER: S-EPMC6401273 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Publications
A versatile image analysis platform for three-dimensional nuclear reconstruction.
Williams Jessica F JF Mochrie Simon G J SGJ King Megan C MC
Methods (San Diego, Calif.) 20181022
Nuclear morphology is indicative of cellular health in many contexts. In order to robustly and quantitatively measure nuclear size and shape, numerous experimental methods leveraging fluorescence microscopy have been developed. While these methods are useful for quantifying two-dimensional morphology, they often fail to accurately represent the three-dimensional structure of the nucleus, thus omitting important spatial and volumetric information. To address the need for a more accurate image ana ...[more]
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