The NAD+ Salvage Pathway Supports PHGDH-Driven Serine Biosynthesis.
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ABSTRACT:
SUBMITTER: Murphy JP
PROVIDER: S-EPMC6402325 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Publications
The NAD<sup>+</sup> Salvage Pathway Supports PHGDH-Driven Serine Biosynthesis.
Murphy J Patrick JP Giacomantonio Michael A MA Paulo Joao A JA Everley Robert A RA Kennedy Barry E BE Pathak Gopal P GP Clements Derek R DR Kim Youra Y Dai Cathleen C Sharif Tanveer T Gygi Steven P SP Gujar Shashi S
Cell reports 20180801 9
NAD<sup>+</sup> is a key metabolic redox cofactor that is regenerated from nicotinamide through the NAD<sup>+</sup> salvage pathway. Here, we find that inhibiting the NAD<sup>+</sup> salvage pathway depletes serine biosynthesis from glucose by impeding the NAD<sup>+</sup>-dependent protein, 3-phosphoglycerate dehydrogenase (PHGDH). Importantly, we find that PHGDH<sup>high</sup> breast cancer cell lines are exquisitely sensitive to inhibition of the NAD<sup>+</sup> salvage pathway. Further, we fi ...[more]
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