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Phosphate-solubilizing Bacillus sp. enhances growth, phosphorus uptake and oil yield of Mentha arvensis L.

ABSTRACT: In the present study, phosphate solubilizing rhizobacterial isolate STJP from the rhizosphere of Stevia rebaudiana was identified as a Bacillus sp. on the basis of phenotypic, biochemical, and 16S rRNA gene sequencing. In addition to phosphate solubilization ability, isolate Bacillus sp. STJP produced a significant quantity of siderophore (16.06 µg/ml) and indole 3-acetic acid (30.59 µg/ml). In the greenhouse experiment, treatment with STJP along with tricalcium phosphate (TCP200) showed significant increase in the plant growth parameters, oil yield and P uptake in M. arvensis as compared to the control plants. Amongst all the treatments, highest oil yield and menthol content were observed when treated with Bacillus sp. STJP?+?TCP200. Hence, an integrated approach of using Bacillus sp. STJP along with TCP can be used to increase the production of menthol and oil yield of M. arvensis. This approach of using fertilizer along with phosphate solubilizing Bacillus sp. worked very well and was more effective in comparison with individual treatment of fertilizer or plant growth promoting rhizobacteria. A combined use of efficient phosphate solubilising bacteria loaded with plant growth promoting characters along with TCP can thus be far effective way for enhancing the yield of crops in a sustainable manner.

SUBMITTER: Prakash J

PROVIDER: S-EPMC6403274 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Phosphate-solubilizing Bacillus sp. enhances growth, phosphorus uptake and oil yield of Mentha arvensis L.

Prakash Jai J Arora Naveen Kumar NK

3 Biotech 20190306 4

In the present study, phosphate solubilizing rhizobacterial isolate STJP from the rhizosphere of Stevia rebaudiana was identified as a Bacillus sp. on the basis of phenotypic, biochemical, and 16S rRNA gene sequencing. In addition to phosphate solubilization ability, isolate Bacillus sp. STJP produced a significant quantity of siderophore (16.06 µg/ml) and indole 3-acetic acid (30.59 µg/ml). In the greenhouse experiment, treatment with STJP along with tricalcium phosphate (T ...[more]

PMID: 30863705

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Project description:The aim of this study was to isolate, characterize and use phosphate solubilizing bacteria to enhance the bioavailability of insoluble Ca-phosphate for wheat plants. For this purpose, 15 phosphorus solubilizing bacteria (PSB) were isolated from wheat rhizospheric soils of Peshawar and southern Punjab region, Pakistan. These isolates were identified using light microscopy and 16S rRNA gene. Among the isolated bacteria, two strains (Pseudomonas sp. MS16 and Enterobacter sp. MS32) were the efficient P solubilizers based on their P solubilization activity determined qualitatively (solubilization index 3.2-5.8) as well as quantitatively (136-280 ?g mL-1). These two strains produced indole-3-acetic acid (25.6-28.1 ?g mL-1), gibberellic acid (2.5-11.8), solubilized zinc compounds (SI 2.8-3.3) and showed nitrogenase and 1-Aminocyclopropane-1-carboxylic acid deaminase activity in vitro. Phosphate solubilization activity of Pseudomonas sp. MS16 was further validated by amplification, sequencing and phylogenetic analysis of glucose dehydrogenase (gcd) gene (LT908484) responsible for P solubilization. Response Surface Methodology for large-scale production was used to find optimal conditions (Temperature 22.5°C, pH 7) for P solubilization. Glucose was found to support higher P solubilization in vitro. In an in vitro experiment, PSB treated wheat seedlings improved germination and seedling vigor (11% increases) as compared to un-inoculated control. Rhizoscanning of these seedlings showed an increase in various root growth parameters. Wheat inoculation with selected strain MS16 showed pronounced effect on grain yield in pot (38.5% increase) and field (17-18% increase) experiments compared to non-inoculated control. Root colonization by PSB through Florescent in situ Hybridization and Confocal Laser Scanning Microscopy confirmed their rhizosphere competence in soil. BOX-PCR confirmed the re-isolated colonies of Pseudomonas sp. MS16. The results indicated that gluconic acid producing Pseudomonas sp. MS16 from un-explored soils may be cost effective and environment friendly candidate to improve plant growth and phosphorous uptake by wheat plants.

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Phosphate-solubilizing Bacillus sp. enhances growth, phosphorus uptake and oil yield of Mentha arvensis L.

Publications

Phosphate-solubilizing <i>Bacillus</i> sp. enhances growth, phosphorus uptake and oil yield of <i>Mentha arvensis</i> L.

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