Ontology highlight
ABSTRACT:
SUBMITTER: Rossi A
PROVIDER: S-EPMC6403382 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Rossi Axel A Dupaty Léa L Aillot Ludovic L Zhang Liang L Gallien Célia C Hallek Michael M Odenthal Margarete M Adriouch Sahil S Salvetti Anna A Büning Hildegard H
Scientific reports 20190306 1
AAV vectors poorly transduce Dendritic cells (DC), a feature invoked to explain AAV's low immunogenicity. However, the reason for this non-permissiveness remained elusive. Here, we performed an in-depth analysis using human monocyte-derived immature DC (iDC) as model. iDC internalized AAV vectors of various serotypes, but even the most efficient serotype failed to transduce iDC above background. Since AAV vectors reached the cell nucleus, we hypothesized that AAV's intracellular processing occur ...[more]