Ontology highlight
ABSTRACT:
SUBMITTER: Joshi PRH
PROVIDER: S-EPMC6404649 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Joshi Pranav R H PRH Cervera Laura L Ahmed Ibrahim I Kondratov Oleksandr O Zolotukhin Sergei S Schrag Joseph J Chahal Parminder S PS Kamen Amine A AA
Molecular therapy. Methods & clinical development 20190216
Despite numerous advancements in production protocols, manufacturing AAV to meet exceptionally high demand (10<sup>16</sup>-10<sup>17</sup> viral genomes [VGs]) in late clinical stages and for eventual systemic delivery poses significant challenges. Here, we report an efficient, simple, scalable, robust AAV5 production process utilizing the most recent modification of the OneBac platform. An increase in volumetric yield of genomic particles by ∼6-fold and functional particles by ∼20-fold was ach ...[more]