Ontology highlight
ABSTRACT:
SUBMITTER: Naqib A
PROVIDER: S-EPMC6404654 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Naqib Ankur A Jeon Trisha T Kunstman Kevin K Wang Weihua W Shen Yiding Y Sweeney Dagmar D Hyde Marieta M Green Stefan J SJ
PeerJ 20190304
Deep sequencing of small subunit ribosomal RNA (SSU rRNA) gene amplicons continues to be the most common approach for characterization of complex microbial communities. PCR amplifications of conserved regions of SSU rRNA genes often employ degenerate pools of primers to enable targeting of a broad spectrum of organisms. One little noticed feature of such degenerate primer sets is the potential for a wide range of melting temperatures between the primer variants. The melting temperature variation ...[more]