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Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure.

ABSTRACT: Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.

SUBMITTER: Fang T

PROVIDER: S-EPMC6405223 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure.

Fang Tao T Lu Xiaotang X Berger Daniel D Gmeiner Christina C Cho Julia J Schalek Richard R Ploegh Hidde H Lichtman Jeff J

Nature methods 20181105 12

Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hipp ...[more]

PMID: 30397326

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