Ontology highlight
ABSTRACT:
SUBMITTER: Muhar M
PROVIDER: S-EPMC6409205 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20180405 6390
Defining direct targets of transcription factors and regulatory pathways is key to understanding their roles in physiology and disease. We combined SLAM-seq [thiol(SH)-linked alkylation for the metabolic sequencing of RNA], a method for direct quantification of newly synthesized messenger RNAs (mRNAs), with pharmacological and chemical-genetic perturbation in order to define regulatory functions of two transcriptional hubs in cancer, BRD4 and MYC, and to interrogate direct responses to BET bromo ...[more]