Dataset Information

Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.).

ABSTRACT: Rice blast, caused by the fungus, Magnaporthe grisea (M. grisea), lead to the decrease of rice yields widely and destructively, threatening global food security. Although many resistant genes had been isolated and identified in various rice varieties, it is still not enough to clearly understand the mechanism of race-specific resistant ability in rice, especially on the protein level. In this research, proteomic methods were employed to analyze the differentially expressed proteins (DEPs) in susceptible rice variety CO39 and its two near isogenic lines (NILs), CN-4a and CN-4b, in response to the infection of two isolates with different pathogenicity, GUY11 and 81278ZB15. A total of 50 DEPs with more than 1.5-fold reproducible change were identified. At 24 and 48 hpi of GUY11, 32 and 16 proteins in CN-4b were up-regulated, among which 16 and five were paralleled with the expression of their corresponding RNAs. Moreover, 13 of 50 DEPs were reported to be induced by M. grisea in previous publications. Considering the phenotypes of the three tested rice varieties, we found that 21 and 23 up-regulated proteins were responsible for the rice resistant ability to the two different blast isolates, 81278ZB15 and GUY11, respectively. Two distinct branches corresponding to GUY11 and 81278ZB15 were observed in the expression and function of the module cluster of DEPs, illuminating that the DEPs could be responsible for race-specific resistant ability in rice. In other words, DEPs in rice are involved in different patterns and functional modules' response to different pathogenic race infection, inducing race-specific resistant ability in rice.

SUBMITTER: Ma S

PROVIDER: S-EPMC6410114 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.).

Ma Shiwei S Lin Shoukai S Wang Menglin M Zou Yang Y Tao Huan H Liu Wei W Zhang Lina L Liang Kangjing K Ai Yufang Y He Huaqin H

Plants (Basel, Switzerland) 20190123 2

Rice blast, caused by the fungus, Magnaporthe grisea (M. grisea), lead to the decrease of rice yields widely and destructively, threatening global food security. Although many resistant genes had been isolated and identified in various rice varieties, it is still not enough to clearly understand the mechanism of race-specific resistant ability in rice, especially on the protein level. In this research, proteomic methods were employed to analyze the differentially expressed proteins ...[more]

PMID: 30678057

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Project description:Rice hull, the outer cover of the rice grain, determines grain shape and size. Changes in the rice hull proteome in different growth stages may reflect the underlying mechanisms involved in grain development. To better understand these changes, isobaric tags for relative and absolute quantitative (iTRAQ) MS/MS was used to detect statistically significant changes in the rice hull proteome in the booting, flowering, and milk-ripe growth stages. Differentially expressed proteins were analyzed to predict their potential functions during development. Gene ontology (GO) terms and pathways were used to evaluate the biological mechanisms involved in rice hull at the three growth stages. In total, 5,268 proteins were detected and characterized, of which 563 were differentially expressed across the development stages. The results showed that the flowering and milk-ripe stage proteomes were more similar to each other (r=0.61) than either was to the booting stage proteome. A GO enrichment analysis of the differentially expressed proteins was used to predict their roles during rice hull development. The potential functions of 25 significantly differentially expressed proteins were used to evaluate their possible roles at various growth stages. Among these proteins, an unannotated protein (Q7X8A1) was found to be overexpressed especially in the flowering stage, while a putative uncharacterized protein (B8BF94) and an aldehyde dehydrogenase (Q9FPK6) were overexpressed only in the milk-ripe stage. Pathways regulated by differentially expressed proteins were also analyzed. Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase (Q9SDJ2), and two magnesium-chelatase subunits, ChlD (Q6ATS0), and ChlI (Q53RM0), were associated with chlorophyll biosynthesis at different developmental stages. The expression of Q9SDJ2 in the flowering and milk-ripe stages was validated by qRT-PCR. The 25 candidate proteins may be pivotal markers for controlling rice hull development at various growth stages and chlorophyll biosynthesis pathway related proteins, especially magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase (Q9SDJ2), may provide new insights into the molecular mechanisms of rice hull development and chlorophyll associated regulation.

Dataset Information

Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.).

Publications

Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (<i>Oryza sativa</i> L.).

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