Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses.
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ABSTRACT:
SUBMITTER: Luo H
PROVIDER: S-EPMC6411097 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Publications
Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses.
Luo Hao H Hansen Anne Sofie L ASL Yang Lei L Schneider Konstantin K Kristensen Mette M Christensen Ulla U Christensen Hanne B HB Du Bin B Özdemir Emre E Feist Adam M AM Keasling Jay D JD Jensen Michael K MK Herrgård Markus J MJ Palsson Bernhard O BO
PLoS biology 20190311 3
We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone ...[more]
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