Ontology highlight
ABSTRACT:
SUBMITTER: Li L
PROVIDER: S-EPMC6411747 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Li Lingting L Fang Chengli C Zhuang Ningning N Wang Tiantian T Zhang Yu Y
Nature communications 20190311 1
Bacterial RNA polymerase employs extra-cytoplasmic function (ECF) σ factors to regulate context-specific gene expression programs. Despite being the most abundant and divergent σ factor class, the structural basis of ECF σ factor-mediated transcription initiation remains unknown. Here, we determine a crystal structure of Mycobacterium tuberculosis (Mtb) RNAP holoenzyme comprising an RNAP core enzyme and the ECF σ factor σ<sup>H</sup> (σ<sup>H</sup>-RNAP) at 2.7 Å, and solve another crystal struc ...[more]