Ontology highlight
ABSTRACT:
SUBMITTER: Savell KE
PROVIDER: S-EPMC6412672 | biostudies-literature | 2019 Jan-Feb
REPOSITORIES: biostudies-literature
Savell Katherine E KE Bach Svitlana V SV Zipperly Morgan E ME Revanna Jasmin S JS Goska Nicholas A NA Tuscher Jennifer J JJ Duke Corey G CG Sultan Faraz A FA Burke Julia N JN Williams Derek D Ianov Lara L Day Jeremy J JJ
eNeuro 20190101 1
CRISPR-based technology has provided new avenues to interrogate gene function, but difficulties in transgene expression in post-mitotic neurons has delayed incorporation of these tools in the central nervous system (CNS). Here, we demonstrate a highly efficient, neuron-optimized dual lentiviral CRISPR-based transcriptional activation (CRISPRa) system capable of robust, modular, and tunable gene induction and multiplexed gene regulation across several primary rodent neuron culture systems. CRISPR ...[more]