Dataset Information

Photocatalytic antibacterial application of zinc oxide nanoparticles and self-assembled networks under dual UV irradiation for enhanced disinfection.

ABSTRACT:

Background

Zinc oxide (ZnO) nanoparticles and their networks have been developed for use in various applications such as gas sensors and semiconductors.

Aim

In this study, their antibacterial activity against Escherichia coli under dual ultraviolet (UV) irradiation for disinfection was investigated.

Materials and methods

ZnO nanoparticles were synthesized and immobilized onto silicon (Si) wafers by self-assembly. The physicochemical properties and antibacterial activity of ZnO nanoparticles and their networks were evaluated. Gene ontology was analyzed and toxicity levels were also monitored.

Results

Synthesized ZnO nanoparticles were spherical nanocrystals (<100 nm; Zn, 47%; O, 53%) that formed macro-mesoporous three-dimensional nanostructures on Si wafers in a concentration-dependent manner. ZnO nanoparticles and their networks on Si wafers had an excellent antibacterial activity against E. coli under dual UV irradiation (>3log CFU/mL). Specifically, arrayed ZnO nanoparticle networks showed superior activity compared with free synthesized ZnO nanoparticles. Oxidative stress-responsive proteins in E. coli were identified and categorized, which indicated antibacterial activity. Synthesized ZnO nanoparticles were less cytotoxic in HaCaT with an IC50 of 6.632 mg/mL, but phototoxic in Balb/c 3T3.

Conclusion

The results suggested that ZnO nanoparticles and their networks can be promising photocatalytic antibiotics for use in next-generation disinfection systems. Their application could also be extended to industrial and clinical use as effective and safe photocatalytic antibiotics.

SUBMITTER: Jin SE

PROVIDER: S-EPMC6413819 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Photocatalytic antibacterial application of zinc oxide nanoparticles and self-assembled networks under dual UV irradiation for enhanced disinfection.

Jin Su-Eon SE Jin Jun Eon JE Hwang Woochul W Hong Seok Won SW

International journal of nanomedicine 20190307

<h4>Background</h4>Zinc oxide (ZnO) nanoparticles and their networks have been developed for use in various applications such as gas sensors and semiconductors.<h4>Aim</h4>In this study, their antibacterial activity against <i>Escherichia coli</i> under dual ultraviolet (UV) irradiation for disinfection was investigated.<h4>Materials and methods</h4>ZnO nanoparticles were synthesized and immobilized onto silicon (Si) wafers by self-assembly. The physicochemical properties and antibacterial activ ...[more]

PMID: 30880977

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Project description:BackgroundDrug-resistant pathogens and industrial dye wastes have emerged as critical global public health concerns, posing significant risks to human and animal health, as well as to environmental sustainability. Green synthesized nano absorbents were found to be a viable strategy for treating drug-resistant pathogens and in wastewater. Hence, this study endeavored the synthesis of piperine-driven nano-zinc oxide (ZnONPs) and evaluated them for antibacterial, antibiofilm, and photocatalytic disinfection potential against multi-drug resistant (MDR) foodborne strains of non-typhoidal Salmonella (NTS). Besides, the dye degradation potential of ZnONPs when exposed to UV, sunlight, and LED lights and their antioxidant capacity were assessed.ResultsInitially, in silico analysis of piperine revealed drug-likeliness with minimal toxicity and strong interaction between piperine and OmpC motifs of Salmonella spp. UV spectroscopy of ZnONPs revealed a prominent absorption peak at 340 nm, while PXRD analysis confirmed the hexagonal wurtzite structure of ZnONPs by exhibiting peaks at 30°, 35.6°, 41.3°, 43.6°, 44.3°, 48°, 53°, 58°, and 59.2°, which corresponded to the lattice planes (102), (110), (103), (200), (112), (004), (104), (210), and (211). Additionally, the TEM images demonstrated predominantly spherical ZnONPs with hexagonal wurtzite crystalline SAED pattern. The minimum inhibitory concentration and minimum bactericidal concentration values (µg/mL) of the ZnONPs were found to be 62.50 and 125, respectively. The ZnONPs were observed to be safe with minimal hemolysis (less than 2%) in chicken RBCs, and no cytopathic effects were observed in the MTT assay using HEK cell lines. The NPs were found to be variably stable (high-end temperatures, proteases, cationic salts, and diverse pH), and were tested safe towards commensal gut lactobacilli. Additionally, in vitro time-kill kinetic assay indicated that the MDR-NTS strains were eliminated after co-incubating with ZnONPs for 6 h. The photocatalytic studies exhibited complete bacterial elimination under visible light at 4 h. Interestingly, the ZnONPs significantly inhibited the biofilm formation in the crystal violet staining assay by MDR-NTS strains (P < 0.001) at 24 and 48 h. Besides, a dose-dependent reducing power assay and 2,2'- azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) assay were exhibited. Moreover, ZnONPs significantly degraded methylene blue, crystal violet, and rhodamine-B under different light sources (sunlight, UV light, and LED).ConclusionsThis study revealed a sustainable one-pot method of synthesizing ZnONPs from piperine, which might be used as a viable antibacterial candidate with antioxidant, antibiofilm, and photocatalytic properties with eco-friendly implications and wastewater treatment.

Dataset Information

Photocatalytic antibacterial application of zinc oxide nanoparticles and self-assembled networks under dual UV irradiation for enhanced disinfection.

Background

Aim

Materials and methods

Results

Conclusion

Publications

Photocatalytic antibacterial application of zinc oxide nanoparticles and self-assembled networks under dual UV irradiation for enhanced disinfection.

Similar Datasets

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