Ontology highlight
ABSTRACT:
SUBMITTER: Naamati A
PROVIDER: S-EPMC6414203 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Naamati Adi A Williamson James C JC Greenwood Edward Jd EJ Marelli Sara S Lehner Paul J PJ Matheson Nicholas J NJ
eLife 20190312
Viruses manipulate host cells to enhance their replication, and the identification of cellular factors targeted by viruses has led to key insights into both viral pathogenesis and cell biology. In this study, we develop an HIV reporter virus (HIV-AFMACS) displaying a streptavidin-binding affinity tag at the surface of infected cells, allowing facile one-step selection with streptavidin-conjugated magnetic beads. We use this system to obtain pure populations of HIV-infected primary human CD4+ T c ...[more]