Dataset Information

A comparison between the abdominal and femoral adipose tissue proteome of overweight and obese women.

ABSTRACT: Body fat distribution is an important determinant of cardiometabolic health. Lower-body adipose tissue (AT) has protective characteristics as compared to upper-body fat, but the underlying depot-differences remain to be elucidated. Here, we compared the proteome and morphology of abdominal and femoral AT. Paired biopsies from abdominal and femoral subcutaneous AT were taken from eight overweight/obese (BMI???28?kg/m2) women with impaired glucose metabolism after an overnight fast. Proteins were isolated and quantified using liquid chromatography-mass spectrometry, and protein expression in abdominal and femoral subcutaneous AT was compared. Moreover, correlations between fat cell size and the proteome of both AT depots were determined. In total, 651 proteins were identified, of which 22 proteins tended to be differentially expressed between abdominal and femoral AT after removal of blood protein signals (p?

SUBMITTER: Vogel MAA

PROVIDER: S-EPMC6414508 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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A comparison between the abdominal and femoral adipose tissue proteome of overweight and obese women.

Vogel M A A MAA Wang P P Bouwman F G FG Hoebers N N Blaak E E EE Renes J J Mariman E C EC Goossens G H GH

Scientific reports 20190312 1

Body fat distribution is an important determinant of cardiometabolic health. Lower-body adipose tissue (AT) has protective characteristics as compared to upper-body fat, but the underlying depot-differences remain to be elucidated. Here, we compared the proteome and morphology of abdominal and femoral AT. Paired biopsies from abdominal and femoral subcutaneous AT were taken from eight overweight/obese (BMI ≥ 28 kg/m<sup>2</sup>) women with impaired glucose metabolism after an overnight fast. Pro ...[more]

PMID: 30862933

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Project description:Although vitamin D deficiency is prevalent among obese individuals, its cause is poorly understood. Few studies have measured vitamin D concentrations in adipose of obese (OB) subjects, and none have included normal weight controls (C). The goal of this study was to investigate whether the relationship between body composition, serum 25-hydroxyvitamin D (25OHD), vitamin D in subcutaneous (SQ) and omental (OM) adipose, and total adipose stores of vitamin D differ among OB and C. Obese women undergoing bariatric surgery and normal-weight women undergoing abdominal surgery for benign gynecologic conditions were enrolled. Subjects had measurements of serum 25OHD by high-performance liquid chromatography (HPLC) and body composition by dual-energy X-ray absorptiometry (DXA). Vitamin D concentrations in SQ and OM adipose were measured by mass spectroscopy. Thirty-six women were enrolled. Serum 25OHD was similar between groups (OB 27?±?2 versus C 26?±?2?ng/mL; p?=?0.71). Adipose vitamin D concentrations were not significantly different in either SQ (OB 34?±?9 versus C 26?±?12?ng/g; p?=?0.63) or OM compartments (OB 51?±?13 versus C 30?±?18?ng/g; p?=?0.37). The distribution of vitamin D between SQ and OM compartments was similar between groups. Serum 25OHD was directly related to adipose vitamin D in both groups. Total body vitamin D stores were significantly greater in OB than in C (2.3?±?0.6 versus 0.4?±?0.8?mg, respectively; p?<?0.01). In summary, although OB had significantly greater total vitamin D stores than C, the relationship between serum 25OHD and fat vitamin D and the overall pattern of distribution of vitamin D between the OM and SQ fat compartments was similar. Our data demonstrate that obese subjects have greater adipose stores of vitamin D. They support the hypotheses that the enlarged adipose mass in obese individuals serves as a reservoir for vitamin D and that the increased amount of vitamin D required to saturate this depot may predispose obese individuals to inadequate serum 25OHD. © 2016 American Society for Bone and Mineral Research.

Dataset Information

A comparison between the abdominal and femoral adipose tissue proteome of overweight and obese women.

Publications

A comparison between the abdominal and femoral adipose tissue proteome of overweight and obese women.

Similar Datasets

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