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Carbon replicas reveal double stranded structure of tight junctions in phase-contrast electron microscopy.

ABSTRACT: Replica-based freeze-fracture and freeze-etching electron microscopy methods provide surface topography information, particularly suited to studying membrane protein complexes in their native context. The fidelity and resolution of metal replicas is limited by the inherent property of metal atoms to crystallize. To overcome the limitations of metal replicas, we combined amorphous carbon replicas with phase-contrast electron microscopy. Using this approach, tight junction intramembrane fibrils were shown to have a double stranded morphology.

SUBMITTER: Krystofiak ES

PROVIDER: S-EPMC6414538 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Carbon replicas reveal double stranded structure of tight junctions in phase-contrast electron microscopy.

Krystofiak Evan S ES Heymann J Bernard JB Kachar Bechara B

Communications biology 20190312

Replica-based freeze-fracture and freeze-etching electron microscopy methods provide surface topography information, particularly suited to studying membrane protein complexes in their native context. The fidelity and resolution of metal replicas is limited by the inherent property of metal atoms to crystallize. To overcome the limitations of metal replicas, we combined amorphous carbon replicas with phase-contrast electron microscopy. Using this approach, tight junction intramembrane fibrils we ...[more]

PMID: 30886907

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