PTMselect: optimization of protein modifications discovery by mass spectrometry.
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ABSTRACT:
SUBMITTER: Perchey RT
PROVIDER: S-EPMC6414543 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Publications
PTMselect: optimization of protein modifications discovery by mass spectrometry.
Perchey Renaud T RT Tonini Laure L Tosolini Marie M Fournié Jean-Jacques JJ Lopez Frédéric F Besson Arnaud A Pont Frédéric F
Scientific reports 20190312 1
Discovery of protein modification sites relies on protein digestion by proteases and mass spectrometry (MS) identification of the modified peptides. Depending on proteases used and target protein sequence, this method yields highly variable coverage of modification sites. We introduce PTMselect, a digestion-simulating software which tailors the optimal set of proteases for discovery of global or targeted modification from any single or multiple proteins. ...[more]
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