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Characterization of a Species-Specific Insulinase-Like Protease in Cryptosporidium parvum.


ABSTRACT: Cryptosporidium parvum is an intracellular protozoan that can cause severe diarrhea in humans and various mammals. Results of a comparative genomic analysis indicated that genes encoding two C. parvum-specific insulinase-like proteases (INS19 and INS20), cgd6_5510 and cgd6_5520, are lost in many Cryptosporidium species. In this study, we provided evidence indicating that cgd6_5510 and cgd6_5520 are fragments of a full gene (cgd6_5520-5510) encoding one insulinase-like protease (INS20-19) that is similar in structure to classic insulinases. We expressed cgd6_5510 in Escherichia coli for antiserum preparation and found the protein (INS19) that was partially degraded. A ~180 kDa protein of INS20-19 was specifically recognized by the polyclonal anti-INS19 antiserum in sporozoite lysate. We observed that INS20-19 is likely a protein with high expression in the apical region of sporozoites, and neutralization of the protein led to a partial reduction of parasite load in HCT-8 and MDBK cell cultures at 24 h. Taken together, our findings support the involvement of INS20-19 in the invasion or early developmental process of C. parvum.

SUBMITTER: Zhang S 

PROVIDER: S-EPMC6415594 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Characterization of a Species-Specific Insulinase-Like Protease in <i>Cryptosporidium parvum</i>.

Zhang Shijing S   Wang Yuping Y   Wu Haizhen H   Li Na N   Jiang Jianlin J   Guo Yaqiong Y   Feng Yaoyu Y   Xiao Lihua L  

Frontiers in microbiology 20190306


<i>Cryptosporidium parvum</i> is an intracellular protozoan that can cause severe diarrhea in humans and various mammals. Results of a comparative genomic analysis indicated that genes encoding two <i>C. parvum</i>-specific insulinase-like proteases (INS19 and INS20), <i>cgd6_5510</i> and <i>cgd6_5520</i>, are lost in many <i>Cryptosporidium</i> species. In this study, we provided evidence indicating that <i>cgd6_5510</i> and <i>cgd6_5520</i> are fragments of a full gene (<i>cgd6_5520-5510</i>)  ...[more]

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