Considerations and quality controls when analyzing cell-free tumor DNA.
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ABSTRACT:
SUBMITTER: Johansson G
PROVIDER: S-EPMC6416156 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Publications
Considerations and quality controls when analyzing cell-free tumor DNA.
Johansson Gustav G Andersson Daniel D Filges Stefan S Li Junrui J Muth Andreas A Godfrey Tony E TE Ståhlberg Anders A
Biomolecular detection and quantification 20190213
Circulating cell-free tumor DNA (ctDNA) is a promising biomarker in cancer. Ultrasensitive technologies enable detection of low (< 0.1%) mutant allele frequencies, a pre-requisite to fully utilize the potential of ctDNA in cancer diagnostics. In addition, the entire liquid biopsy workflow needs to be carefully optimized to enable reliable ctDNA analysis. Here, we discuss important considerations for ctDNA detection in plasma. We show how each experimental step can easily be evaluated using simpl ...[more]
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