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Evaluation of the Serologic Cross-Reactivity between Transmissible Gastroenteritis Coronavirus and Porcine Respiratory Coronavirus Using Commercial Blocking Enzyme-Linked Immunosorbent Assay Kits.

ABSTRACT: This study compared the performances of three commercial transmissible gastroenteritis virus/porcine respiratory coronavirus (TGEV/PRCV) blocking enzyme-linked immunosorbent assays (ELISAs) using serum samples (n?=?528) collected over a 49-day observation period from pigs inoculated with TGEV strain Purdue (n?=?12), TGEV strain Miller (n?=?12), PRCV (n = 12), or with virus-free culture medium (n?=?12). ELISA results were evaluated both with "suspect" results interpreted as positive and then as negative. All commercial kits showed excellent diagnostic specificity (99 to 100%) when testing samples from pigs inoculated with virus-free culture medium. However, analyses revealed differences between the kits in diagnostic sensitivity (percent TGEV- or PRCV-seropositive pigs), and all kits showed significant (P?

SUBMITTER: Magtoto R 

PROVIDER: S-EPMC6416363 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Evaluation of the Serologic Cross-Reactivity between Transmissible Gastroenteritis Coronavirus and Porcine Respiratory Coronavirus Using Commercial Blocking Enzyme-Linked Immunosorbent Assay Kits.

Magtoto Ronaldo R   Poonsuk Korakrit K   Baum David D   Zhang Jianqiang J   Chen Qi Q   Ji Ju J   Piñeyro Pablo P   Zimmerman Jeffrey J   Giménez-Lirola Luis G LG  

mSphere 20190313 2

This study compared the performances of three commercial transmissible gastroenteritis virus/porcine respiratory coronavirus (TGEV/PRCV) blocking enzyme-linked immunosorbent assays (ELISAs) using serum samples (<i>n</i> = 528) collected over a 49-day observation period from pigs inoculated with TGEV strain Purdue (<i>n</i> = 12), TGEV strain Miller (<i>n</i> = 12), PRCV (<i>n</i> = 12), or with virus-free culture medium (<i>n</i> = 12). ELISA results were evaluated both with "suspect" results in  ...[more]

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