Dataset Information

Apple consumption is associated with a distinctive microbiota, proteomics and metabolomics profile in the gut of Dawley Sprague rats fed a high-fat diet.

ABSTRACT: Apples contain bioactive compounds with the potential to alleviate clinical signs associated with obesity, a phenomenon likely related to the composition and function of the gut microbiota. The aim of this study was to investigate the effect of apple supplementation on the fecal microbiota and gut metabolites of Dawley Sprague rats fed a high-fat (HF group) or a low-fat (LF group) diet. The fecal microbiota was examined using 16S marker sequencing targeting the V4 region in a MiSeq instrument (Illumina). With the exception of Blautia, which was higher in supplemented rats compared to controls within the LF group, significant differences in fecal microbiota between supplemented rats and controls were only found in the HF group. This suggests that the effect of apple supplementation on the gut microbiota is strongly dependent on the composition of the diet, a phenomenon with potential consequences for obese human patients. Principal Coordinate Analysis of unweighted UniFrac distances revealed a clear strong separation of bacterial communities based on diet (HF and LF, P = 0.001, R = 0.69, ANOSIM test) and based on apple supplementation within the HF group, albeit less strongly (P = 0.006, R = 0.27, ANOSIM test). No differences were found for fecal SCFAs but proteomics and metabolomics analyses showed differential expression of both proteins and metabolites between supplemented rats and controls in the HF group. The results of this study can guide future explorations of the effect of apple supplementation on human health.

SUBMITTER: Garcia-Mazcorro JF

PROVIDER: S-EPMC6417679 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Apple consumption is associated with a distinctive microbiota, proteomics and metabolomics profile in the gut of Dawley Sprague rats fed a high-fat diet.

Garcia-Mazcorro Jose F JF Pedreschi Romina R Yuan Jialing J Kawas Jorge R JR Chew Boon B Dowd Scot E SE Noratto Giuliana G

PloS one 20190314 3

Apples contain bioactive compounds with the potential to alleviate clinical signs associated with obesity, a phenomenon likely related to the composition and function of the gut microbiota. The aim of this study was to investigate the effect of apple supplementation on the fecal microbiota and gut metabolites of Dawley Sprague rats fed a high-fat (HF group) or a low-fat (LF group) diet. The fecal microbiota was examined using 16S marker sequencing targeting the V4 region in a MiSeq instrument (I ...[more]

PMID: 30870465

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Project description:Thyroid hormone mimetics are alluring potential therapies for diseases like dyslipidemia, nonalcoholic fatty liver disease (NAFLD), and insulin resistance. Though diiodothyronines are thought inactive, pharmacologic treatment with 3,5- Diiodo-L-Thyronine (T2) reportedly reduces hepatic lipid content and improves glucose tolerance in fat-fed male rats. To test this, male Sprague Dawley rats fed a safflower-oil based high-fat diet were treated with T2 (0.25 mg/kg-d) or vehicle. Neither 10 nor 30 days of T2 treatment had an effect on weight, adiposity, plasma fatty acids, or hepatic steatosis. Insulin action was quantified in vivo by a hyperinsulinemic-euglycemic clamp. T2 did not alter fasting plasma glucose or insulin concentration. Basal endogenous glucose production (EGP) rate was unchanged. During the clamp, there was no difference in insulin stimulated whole body glucose disposal. Insulin suppressed EGP by 60% ± 10 in T2-treated rats as compared with 47% ± 4 suppression in the vehicle group (p = 0.32). This was associated with an improvement in hepatic insulin signaling; insulin stimulated Akt phosphorylation was ~2.5 fold greater in the T2-treated group as compared with the vehicle-treated group (p = 0.003). There was no change in expression of genes thought to mediate the effect of T2 on hepatic metabolism, including genes that regulate hepatic lipid oxidation (ppara, carnitine palmitoyltransferase 1a), genes that regulate hepatic fatty acid synthesis (srebp1c, acetyl coa carboxylase, fatty acid synthase), and genes involved in glycolysis and gluconeogenesis (L-pyruvate kinase, glucose 6 phosphatase). Therefore, in contrast with previous reports, in Sprague Dawley rats fed an unsaturated fat diet, T2 administration failed to improve NAFLD or whole body insulin sensitivity. Though there was a modest improvement in hepatic insulin signaling, this was not associated with significant differences in hepatic insulin action. Further study will be necessary before diiodothyronines can be considered an effective treatment for NAFLD and dyslipidemia.

Dataset Information

Apple consumption is associated with a distinctive microbiota, proteomics and metabolomics profile in the gut of Dawley Sprague rats fed a high-fat diet.

Publications

Apple consumption is associated with a distinctive microbiota, proteomics and metabolomics profile in the gut of Dawley Sprague rats fed a high-fat diet.

Similar Datasets

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