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DNA methylome analysis provides evidence that the expansion of the tea genome is linked to TE bursts.

ABSTRACT: DNA methylation is essential for gene regulation, imprinting and silencing of transposable elements (TEs). Although bursts of transposable elements are common in many plant lineages, how plant DNA methylation is related to transposon bursts remains unclear. Here we explore the landscape of DNA methylation of tea, a species thought to have experienced a recent transposon burst event. This species possesses more transposable elements than any other sequenced asterids (potato, tomato, coffee, pepper and tobacco). The overall average DNA methylation levels were found to differ among the tea, potato and tomato genomes, and methylation at CHG sequence sites was found to be significantly higher in tea than that in potato or tomato. Moreover, the abundant TEs resulting from burst events not only resulted in tea developing a very large genome size, but also affected many genes involved in importantly biological processes, including caffeine, theanine and flavonoid metabolic pathway genes. In addition, recently transposed TEs were more heavily methylated than ancient ones, implying that DNA methylation is proportionate to the degree of TE silencing, especially on recent active ones. Taken together, our results show that DNA methylation regulates transposon silencing and may play a role in genome size expansion.

SUBMITTER: Wang L

PROVIDER: S-EPMC6419580 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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DNA methylome analysis provides evidence that the expansion of the tea genome is linked to TE bursts.

Wang Lei L Shi Yan Y Chang Xiaojun X Jing Shengli S Zhang Qunjie Q You Chenjiang C Yuan Hongyu H Wang Haifeng H

Plant biotechnology journal 20181015 4

DNA methylation is essential for gene regulation, imprinting and silencing of transposable elements (TEs). Although bursts of transposable elements are common in many plant lineages, how plant DNA methylation is related to transposon bursts remains unclear. Here we explore the landscape of DNA methylation of tea, a species thought to have experienced a recent transposon burst event. This species possesses more transposable elements than any other sequenced asterids (potato, tomato, coffee, peppe ...[more]

PMID: 30256509

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DNA methylome analysis provides evidence that the expansion of the tea genome is linked to TE bursts.

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DNA methylome analysis provides evidence that the expansion of the tea genome is linked to TE bursts.

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