Molecular mechanistic insight into the anti-hyperuricemic effect of Eucommia ulmoides in mice and rats.

ABSTRACT: CONTEXT:Eucommia ulmoides Oliver (Eucommiaceae) has various medicinal properties. Our previous studies revealed that Eucommia ulmoides has a protective effect on hyperuricaemia. OBJECTIVE:This study investigates the effect of Eucommia ulmoides cortex ethanol extract (EU) on hyperuricaemia and explores the underlying mechanism in Kunming mice and Sprague-Dawley rats. MATERIAL AND METHODS:Sixty mice and sixty rats were divided into normal control, hyperuricaemia, allopurinol (10?mg/kg) and three EU groups. The EU groups received intragastric EU at 80, 160, 320?mg/kg in mice and 100, 200, 400?mg/kg in rats for 7?days. Serum uric acid (SUA) was measured using a kit. mRNA and proteins were quantified by RT-qPCR and immunohistochemical assays (IHC), respectively. RESULTS:The Maximal Tolerable Dose (MTD) of EU administered intragastrically was 18?g/kg in mice. The intermediate (160?mg/kg) and high (320?mg/kg) EU treatment significantly reduced (p?

SUBMITTER: Fang C 

PROVIDER: S-EPMC6419643 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature