Dataset Information

Acetate-induced modulation of ascorbate: glutathione cycle and restriction of sodium accumulation in shoot confer salt tolerance in Lens culinaris Medik.

ABSTRACT: Physiological and biochemical changes in six-day-old hydroponically grown lentil seedlings exposed to 100 mM salinity stress with or without 5 and 10 mM Na-acetate were studied. Results showed that salt stress reduced recovery percentage, fresh weight (FW), chlorophyll (chl) content, disturbed water balance, disrupted antioxidant defense pathway by decreasing reduced ascorbate content, and caused ion toxicity resulting from increased Na⁺ accumulation, severe K⁺ loss from roots in hydroponic culture. However, exogenous application of Na-acetate improved the seedling growth by maintaining water balance and increasing chl content. Furthermore, Na-acetate application reduced oxidative damage by modulating antioxidant defense pathway, and sustained ion homeostasis by reducing Na⁺ uptake and K⁺ loss. In the second experiment in glass house, we investigated the role of Na-acetate on lentil for long-term salinity. Acetate application increased FW and dry weight, reduced oxidative and membrane damage, and lowered the accumulation of Na⁺ in shoot compared with salt stressed seedlings alone. From the results of both experiments, it is clear that the exogenous application of Na-acetate enhanced salt tolerance in lentil seedlings.

SUBMITTER: Hossain MS

PROVIDER: S-EPMC6419701 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Acetate-induced modulation of ascorbate: glutathione cycle and restriction of sodium accumulation in shoot confer salt tolerance in Lens culinaris Medik.

Hossain Md Shahadat MS Hasanuzzaman Mirza M Sohag Md Mahmodul Hasan MMH Bhuyan M H M Borhannuddin MHMB Fujita Masayuki M

Physiology and molecular biology of plants : an international journal of functional plant biology 20190206 2

Physiological and biochemical changes in six-day-old hydroponically grown lentil seedlings exposed to 100 mM salinity stress with or without 5 and 10 mM Na-acetate were studied. Results showed that salt stress reduced recovery percentage, fresh weight (FW), chlorophyll (chl) content, disturbed water balance, disrupted antioxidant defense pathway by decreasing reduced ascorbate content, and caused ion toxicity resulting from increased Na+ accumulation, severe K+ loss from ro ...[more]

PMID: 30956427

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Project description:Frost is one of the main abiotic stresses limiting plant distribution and crop production. To cope with the stress, plants evolved adaptations known as cold acclimation or chilling tolerance to maximize frost tolerance. Cold acclimation is a progressive acquisition of freezing tolerance by plants subjected to low non-freezing temperatures which subsequently allows them to survive exposure to frost. Lentil is a cool season grain legume that is challenged by winter frost in some areas of its cultivation.To better understand the genetic base of frost tolerance differential gene expression in response to cold acclimation was investigated. Recombinant inbred lines (RILs) from the cross Precoz x WA8649041 were first classified as cold tolerant or cold susceptible according to their response to temperatures between -3 to -15 °C. Then, RILs from both extremes of the response curve were cold acclimated and the leaf transcriptomes of two bulks each of eight frost tolerant and seven cold susceptible RILs were investigated by Deep Super-SAGE transcriptome profiling. Thus, four RNA bulks were analysed: the acclimated susceptible, the acclimated tolerant and the respective controls (non-acclimated susceptible and non-acclimated tolerant). Approximately 16.5 million 26 nucleotide long Super-SAGE tags were sequenced in the four sets (between ~3 and 5.4 millions). In total, 133,077 different unitags, each representing a particular transcript isoform, were identified in these four sets. Tags which showed a significantly different abundance in any of the bulks (fold change ?4.0 and a significant p-value <0.001) were selected and used to identify the corresponding lentil gene sequence. Three hundred of such lentil sequences were identified. Most of their known homologs coded for glycine-rich, cold and drought-regulated proteins, dormancy-associated proteins, proline-rich proteins (PRPs) and other membrane proteins. These were generally but not exclusively over-expressed in the acclimated tolerant lines.This set of candidate genes implicated in the response to frost in lentil represents an useful base for deeper and more detailed investigations into this important agronomic trait in future.

Dataset Information

Acetate-induced modulation of ascorbate: glutathione cycle and restriction of sodium accumulation in shoot confer salt tolerance in Lens culinaris Medik.

Publications

Acetate-induced modulation of ascorbate: glutathione cycle and restriction of sodium accumulation in shoot confer salt tolerance in <i>Lens culinaris</i> Medik.

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