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Noninvasive monitoring of single-cell mechanics by acoustic scattering.

ABSTRACT: The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of?

SUBMITTER: Kang JH

PROVIDER: S-EPMC6420125 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Noninvasive monitoring of single-cell mechanics by acoustic scattering.

Kang Joon Ho JH Miettinen Teemu P TP Chen Lynna L Olcum Selim S Katsikis Georgios G Doyle Patrick S PS Manalis Scott R SR

Nature methods 20190211 3

The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized acoustic scattering (SNACS), measures stiffness. To demonstr ...[more]

PMID: 30742041

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