Ontology highlight
ABSTRACT:
SUBMITTER: Pereira A
PROVIDER: S-EPMC6420153 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Pereira António A Sousa Mafalda M Almeida Ana C AC Ferreira Luísa T LT Costa Ana Rita AR Novais-Cruz Marco M Ferrás Cristina C Sousa Mónica Mendes MM Sampaio Paula P Belsley Michael M Maiato Helder H
Optics express 20190305 6
Stimulated emission depletion (STED) fluorescence microscopy squeezes an excited spot well below the wavelength scale using a doughnut-shaped depletion beam. To generate a doughnut, a scale-free vortex phase modulation (2D-STED) is often used because it provides maximal transverse confinement and radial-aberration immunity (RAI) to the central dip. However, RAI also means blindness to a defocus term, making the axial origin of fluorescence photons uncertain within the wavelength scale provided b ...[more]