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Self-digitization chip for quantitative detection of human papillomavirus gene using digital LAMP.

ABSTRACT: Digital nucleic acid amplification and detection methods provide excellent sensitivity and specificity and allow absolute quantification of target nucleic acids. Isothermal methods such as digital loop-mediated isothermal amplification (digital LAMP) have potential for use in rapid disease diagnosis in low-resource settings due to their speed and lack of thermal cycling. We previously developed a self-digitization (SD) chip, a simple microfluidics device that automatically digitizes a sample into an array of nanoliter wells, for use in digital LAMP. In this work, we improve the SD chip design to increase sample loading efficiency, speed, and completeness, and test a range of well volumes and numbers. We demonstrate the diagnostic capability of this platform by applying it to quantifying human papillomavirus 18 gene.

SUBMITTER: Kreutz JE

PROVIDER: S-EPMC6420227 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Self-digitization chip for quantitative detection of human papillomavirus gene using digital LAMP.

Kreutz Jason E JE Wang Jiasi J Sheen Allison M AM Thompson Alison M AM Staheli Jeannette P JP Dyen Michael R MR Feng Qinghua Q Chiu Daniel T DT

Lab on a chip 20190301 6

Digital nucleic acid amplification and detection methods provide excellent sensitivity and specificity and allow absolute quantification of target nucleic acids. Isothermal methods such as digital loop-mediated isothermal amplification (digital LAMP) have potential for use in rapid disease diagnosis in low-resource settings due to their speed and lack of thermal cycling. We previously developed a self-digitization (SD) chip, a simple microfluidics device that automatically digitizes a sample int ...[more]

PMID: 30734822

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