Ontology highlight
ABSTRACT:
SUBMITTER: Pankowicz FP
PROVIDER: S-EPMC6420307 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Pankowicz Francis P FP Barzi Mercedes M Kim Kang Ho KH Legras Xavier X Martins Celeste Santos CS Wooton-Kee Clavia Ruth CR Lagor William R WR Marini Juan C JC Elsea Sarah H SH Bissig-Choisat Beatrice B Moore David D DD Bissig Karl-Dimiter KD
Gastroenterology 20180828 6
<h4>Background & aims</h4>Despite advances in gene editing technologies, generation of tissue-specific knockout mice is time-consuming. We used CRISPR/Cas9-mediated genome editing to disrupt genes in livers of adult mice in just a few months, which we refer to as somatic liver knockouts.<h4>Methods</h4>In this system, Fah<sup>-/-</sup> mice are given hydrodynamic tail vein injections of plasmids carrying CRISPR/Cas9 designed to excise exons in Hpd; the Hpd-edited hepatocytes have a survival adva ...[more]