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Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development.


ABSTRACT:

Purpose

Motility of spermatozoa helps not only in planning the type of infertility treatment but also directly reflects the success rate in assisted reproductive technology (ART). Previously, biotin, a water-soluble vitamin, has been shown to increase the motility and longevity of cryopreserved human spermatozoa. The present study was designed to understand the molecular basis of the beneficial effects of presence of biotin in sperm wash medium on early embryo development.

Methods

The effect biotin supplementation to sperm wash medium on the sperm parameters were assessed in swim-up fraction of normozoospermic and asthenozoospermic ejaculates collected from infertile men. Fertilization and early embryo development was studied using Swiss albino mice.

Results

Even though both biotin and pentoxifylline (PTX) enhanced the motility of spermatozoa from normozoospermic and asthenozoospermic samples, biotin group exhibited higher in vitro survival. Using mouse model, we observed that presence of biotin or PTX in sperm wash medium improved the fertilization rate and blastocyst rate compared to control. Blastocysts from these groups had significantly higher total cell number (P?ConclusionOur results indicate that presence of biotin in sperm wash medium can improve the fertilization potential and preimplantation embryo development and can be considered as a safe alternate to PTX.

SUBMITTER: Salian SR 

PROVIDER: S-EPMC6420554 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development.

Salian Sujith Raj SR   Nayak Guruprasad G   Kumari Sandhya S   Patel Sandesh S   Gowda Shruthi S   Shenoy Yashaswini Y   Sugunan Sinoy S   G K Rajanikant R   Managuli Renuka Suresh RS   Mutalik Srinivas S   Dahiya Vandana V   Pal Samanwita S   Adiga Satish Kumar SK   Kalthur Guruprasad G  

Journal of assisted reproduction and genetics 20181004 2


<h4>Purpose</h4>Motility of spermatozoa helps not only in planning the type of infertility treatment but also directly reflects the success rate in assisted reproductive technology (ART). Previously, biotin, a water-soluble vitamin, has been shown to increase the motility and longevity of cryopreserved human spermatozoa. The present study was designed to understand the molecular basis of the beneficial effects of presence of biotin in sperm wash medium on early embryo development.<h4>Methods</h4  ...[more]

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