Dataset Information

Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection.

ABSTRACT: Tomato leaf curl virus (ToLCV) belonging to Begomovirus family of Geminivirus is known to cause one of the most destructive diseases in tomato. Amongst various ToLCVs, a monopartite Tomato leaf curl Joydebpur virus (ToLCJoV) is most prevalent in eastern part of India. In the present study, we observed induced expression of one of the negative regulators of ethylene signaling pathway gene (LeCTR1) in ToLCJoV infected plants. The Tobacco rattle virus (TRV) induced silencing of the LeCTR1 gene provided enhanced tolerance to ToLCJoV infections. The leaf curling as well as ROS accumulation was significantly reduced in the viral infected LeCTR1 silenced plants. Induction of several defense marker genes (NPR1, PR1, PR5, AOS2, EIN2, EIN3 and ERF5) reinforced enhanced tolerance against ToLCJoV infection in the LeCTR1 silenced tomato. Overall, the present study provides evidence that silencing of LeCTR1 can be deployed to protect tomato from ToLCJoV infections.

SUBMITTER: Chandan RK

PROVIDER: S-EPMC6422369 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection.

Chandan Ravindra K RK Singh Achuit K AK Patel Sunita S Swain Durga Madhab DM Tuteja Narendra N Jha Gopaljee G

Plant signaling & behavior 20190119 3

Tomato leaf curl virus (ToLCV) belonging to Begomovirus family of Geminivirus is known to cause one of the most destructive diseases in tomato. Amongst various ToLCVs, a monopartite Tomato leaf curl Joydebpur virus (ToLCJoV) is most prevalent in eastern part of India. In the present study, we observed induced expression of one of the negative regulators of ethylene signaling pathway gene (LeCTR1) in ToLCJoV infected plants. The Tobacco rattle virus (TRV) induced silencing of the LeCTR1 gene prov ...[more]

PMID: 30661468

Similar Datasets

Project description:BackgroundGeminiviruses are DNA plant viruses that cause highly damaging diseases affecting crops worldwide. During the infection, geminiviruses hijack cellular processes, suppress plant defenses, and cause a massive reprogramming of the infected cells leading to major changes in the whole plant homeostasis. The advances in sequencing technologies allow the simultaneous analysis of multiple aspects of viral infection at a large scale, generating new insights into the molecular mechanisms underlying plant-virus interactions. However, an integrative study of the changes in the host transcriptome, small RNA profile and methylome during a geminivirus infection has not been performed yet. Using a time-scale approach, we aim to decipher the gene regulation in tomato in response to the infection with the geminivirus, tomato yellow leaf curl virus (TYLCV).ResultsWe showed that tomato undergoes substantial transcriptional and post-transcriptional changes upon TYLCV infection and identified the main altered regulatory pathways. Interestingly, although the principal plant defense-related processes, gene silencing and the immune response were induced, this cannot prevent the establishment of the infection. Moreover, we identified extra- and intracellular immune receptors as targets for the deregulated microRNAs (miRNAs) and established a network for those that also produced phased secondary small interfering RNAs (phasiRNAs). On the other hand, there were no significant genome-wide changes in tomato methylome at 14 days post infection, the time point at which the symptoms were general, and the amount of viral DNA had reached its maximum level, but we were able to identify differentially methylated regions that could be involved in the transcriptional regulation of some of the differentially expressed genes.ConclusionWe have conducted a comprehensive and reliable study on the changes at transcriptional, post-transcriptional and epigenetic levels in tomato throughout TYLCV infection. The generated genomic information is substantial for understanding the genetic, molecular and physiological changes caused by TYLCV infection in tomato.

Dataset Information

Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection.

Publications

Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets