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Regulation of plasma membrane H+-ATPase activity by the members of the V-SNARE VAMP7C family in arabidopsis thaliana.

ABSTRACT: The plasma membrane (PM) H+-ATPase plays an important role in response to environmental stress, such as salt-alkaline stress. We have reported that the N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein Vesicle-associated membrane protein 711 (VAMP711) is involved in drought stress by regulating PM H+-ATPase activity directly. In this study, we report that VAMP7C (VAMP711-14) represses PM H+-ATPase activity in response to high-pH stress. The Arabidopsis PM H+-ATPase AHA2 interacts with VAMP7C. The longin domain of VAMP711 interacts with AHA2 to inhibit PM H+-ATPase activity on the plasma membrane. The alkaline phenotype of vamp711 and vamp711 vamp712 vamp713 triple mutants suggested that VAMP7C was functionally redundant in regulating PM H+-ATPase activity in response to high-pH stress.

SUBMITTER: Xue Y

PROVIDER: S-EPMC6422389 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Regulation of plasma membrane H+-ATPase activity by the members of the V-SNARE VAMP7C family in arabidopsis thaliana.

Xue Yuan Y Zhao Shuangshuang S Yang Zhijia Z Guo Yan Y Yang Yongqing Y

Plant signaling & behavior 20190205 3

The plasma membrane (PM) H+-ATPase plays an important role in response to environmental stress, such as salt-alkaline stress. We have reported that the N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein Vesicle-associated membrane protein 711 (VAMP711) is involved in drought stress by regulating PM H+-ATPase activity directly. In this study, we report that VAMP7C (VAMP711-14) represses PM H+-ATPase activity in response to high-pH stress ...[more]

PMID: 30720384

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