Dataset Information

Radiation-induced astrocyte senescence is rescued by ?133p53.

ABSTRACT:

Background

Cellular senescence and the senescence-associated secretory phenotype (SASP) may contribute to the development of radiation therapy-associated side effects in the lung and blood vessels by promoting chronic inflammation. In the brain, inflammation contributes to the development of neurologic disease, including Alzheimer's disease. In this study, we investigated the roles of cellular senescence and ?133p53, an inhibitory isoform of p53, in radiation-induced brain injury.

Methods

Senescent cell types in irradiated human brain were identified with immunohistochemical labeling of senescence-associated proteins p16INK4A and heterochromatin protein Hp1? in 13 patient cases, including 7 irradiated samples. To investigate the impact of radiation on astrocytes specifically, primary human astrocytes were irradiated and examined for expression of ?133p53 and induction of SASP. Lentiviral expression of ?133p53 was performed to investigate its role in regulating radiation-induced cellular senescence and astrocyte-mediated neuroinflammation.

Results

Astrocytes expressing p16INK4A and Hp1? were identified in all irradiated tissues, were increased in number in irradiated compared with untreated cancer patient tissues, and had higher labeling intensity in irradiated tissues compared with age-matched controls. Human astrocytes irradiated in vitro also experience induction of cellular senescence, have diminished ?133p53, and adopt a neurotoxic phenotype as demonstrated by increased senescence-associated beta-galactosidase activity, p16INK4A, and interleukin (IL)-6. In human astrocytes, ?133p53 inhibits radiation-induced senescence, promotes DNA double-strand break repair, and prevents astrocyte-mediated neuroinflammation and neurotoxicity.

Conclusions

Restoring expression of the endogenous p53 isoform, ?133p53, protects astrocytes from radiation-induced senescence, promotes DNA repair, and inhibits astrocyte-mediated neuroinflammation.

SUBMITTER: Turnquist C

PROVIDER: S-EPMC6422440 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Publications

Radiation-induced astrocyte senescence is rescued by Δ133p53.

Turnquist Casmir C Beck Jessica A JA Horikawa Izumi I Obiorah Ifeyinwa E IE Von Muhlinen Natalia N Vojtesek Borivoj B Lane David P DP Grunseich Christopher C Chahine Joeffrey J JJ Ames Heather M HM Smart Dee Dee DD Harris Brent T BT Harris Curtis C CC

Neuro-oncology 20190301 4

<h4>Background</h4>Cellular senescence and the senescence-associated secretory phenotype (SASP) may contribute to the development of radiation therapy-associated side effects in the lung and blood vessels by promoting chronic inflammation. In the brain, inflammation contributes to the development of neurologic disease, including Alzheimer's disease. In this study, we investigated the roles of cellular senescence and Δ133p53, an inhibitory isoform of p53, in radiation-induced brain injury.<h4>Met ...[more]

PMID: 30615147

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