Ontology highlight
ABSTRACT:
SUBMITTER: Horvath G
PROVIDER: S-EPMC6423008 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Horváth Gergő G Egyed Orsolya O Tang Changguo C Kovács Mihály M Micsonai András A Kardos József J Toke Orsolya O
Scientific reports 20190318 1
Human ileal bile acid-binding protein (hI-BABP) has a key role in the intracellular transport of bile salts. To explore the role of histidine protonation in the binding process, the pH-dependence of bile salt binding and internal dynamics in hI-BABP was investigated using NMR spectroscopy and biophysical tools. Thermodynamic and kinetic measurements show an increase in the overall binding affinity and the association rate constant of the first binding step below the pK<sub>a</sub> of the histidi ...[more]