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Transgenic tomatoes for abiotic stress tolerance: status and way ahead.

ABSTRACT: Tomato (Solanum lycopersicum) is one of the most important vegetable crops; its production, productivity and quality are adversely affected by abiotic stresses. Abiotic stresses such as drought, extreme temperature and high salinity affect almost every stage of tomato life cycle. Depending upon the plant stage and duration of the stress, abiotic stress causes about 70% yield loss. Several wild tomato species have the stress tolerance genes; however, it is very difficult to transfer them into cultivars due to high genetic distance and crossing barriers. Transgenic technology is an alternative potential tool for the improvement of tomato crop to cope with abiotic stress, as it allows gene transfer across species. In recent decades, many transgenic tomatoes have been developed, and many more are under progress against abiotic stress using transgenes such as DREBs, Osmotin, ZAT12 and BADH2. The altered expression of these transgenes under abiotic stresses are involved in every step of stress responses, such as signaling, control of transcription, proteins and membrane protection, compatible solute (betaines, sugars, polyols, and amino acids) synthesis, and free-radical and toxic-compound scavenging. The stress-tolerant transgenic tomato development is based on introgression of a gene with known function in stress response and putative tolerance. Transgenic tomato plants have been developed against drought, heat and salt stress with the help of various transgenes, expression of which manages the stress at the cellular level by modulating the expression of downstream genes to ultimately improve growth and yield of tomato plants and help in sustainable agricultural production. The transgenic technology could be a faster way towards tomato improvement against abiotic stress. This review provides comprehensive information about transgenic tomato development against abiotic stress such as drought, heat and salinity for researcher attention and a better understanding of transgenic technology used in tomato improvement and sustainable agricultural production.

SUBMITTER: Krishna R

PROVIDER: S-EPMC6423223 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Transgenic tomatoes for abiotic stress tolerance: status and way ahead.

Krishna Ram R Karkute Suhas G SG Ansari Waquar A WA Jaiswal Durgesh Kumar DK Verma Jay Prakash JP Singh Major M

3 Biotech 20190318 4

Tomato (<i>Solanum lycopersicum</i>) is one of the most important vegetable crops; its production, productivity and quality are adversely affected by abiotic stresses. Abiotic stresses such as drought, extreme temperature and high salinity affect almost every stage of tomato life cycle. Depending upon the plant stage and duration of the stress, abiotic stress causes about 70% yield loss. Several wild tomato species have the stress tolerance genes; however, it is very difficult to transfer them i ...[more]

PMID: 30944790

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Project description:BACKGROUND: The pepper fruit is the second most consumed vegetable worldwide. However, low temperature affects the vegetative development and reproduction of the pepper, resulting in economic losses. To identify cold-related genes regulated by abscisic acid (ABA) in pepper seedlings, cDNA representational difference analysis was previously performed using a suppression subtractive hybridization method. One of the genes cloned from the subtraction was homologous to Solanum tuberosum MBF1 (StMBF1) encoding the coactivator multiprotein bridging factor 1. Here, we have characterized this StMBF1 homolog (named CaMBF1) from Capsicum annuum and investigated its role in abiotic stress tolerance. RESULTS: Tissue expression profile analysis using quantitative RT-PCR showed that CaMBF1 was expressed in all tested tissues, and high-level expression was detected in the flowers and seeds. The expression of CaMBF1 in pepper seedlings was dramatically suppressed by exogenously supplied salicylic acid, high salt, osmotic and heavy metal stresses. Constitutive overexpression of CaMBF1 in Arabidopsis aggravated the visible symptoms of leaf damage and the electrolyte leakage of cell damage caused by cold stress in seedlings. Furthermore, the expression of RD29A, ERD15, KIN1, and RD22 in the transgenic plants was lower than that in the wild-type plants. On the other hand, seed germination, cotyledon greening and lateral root formation were more severely influenced by salt stress in transgenic lines compared with wild-type plants, indicating that CaMBF1-overexpressing Arabidopsis plants were hypersensitive to salt stress. CONCLUSIONS: Overexpression of CaMBF1 in Arabidopsis displayed reduced tolerance to cold and high salt stress during seed germination and post-germination stages. CaMBF1 transgenic Arabidopsis may reduce stress tolerance by downregulating stress-responsive genes to aggravate the leaf damage caused by cold stress. CaMBF1 may be useful for genetic engineering of novel pepper cultivars in the future.

Dataset Information

Transgenic tomatoes for abiotic stress tolerance: status and way ahead.

Publications

Transgenic tomatoes for abiotic stress tolerance: status and way ahead.

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